For the Human Immunoglobulins

Human Isotype EIA

Capture Antibody

Detection Antibody

Total Human IgG

HP6046P

HP6043-HRP

Total Human IgG

HP6046P

PolyMono anti-human IgG *

Total Human IgG1

HP6069P

PolyMono anti-human IgG*

   

Anti-Kappa/Lambda MAb *

Total Human IgG2

HP6002P

PolyMono anti-human IgG*

   

Anti-Kappa/Lambda MAb *

Total Human IgG3

HP6047P

PolyMono anti-human IgG*

   

Anti-Kappa/Lambda MAb *

Total Human IgG4

HP6025P

PolyMono anti-human IgG*

   

Anti-Kappa/Lambda MAb *

Total Human IgE

HP6061P

HP6029-Biotin

Total Human IgA

HP6111P

HP6123-Biotin

   

HP6123-HRP

Total Secretory IgA

HP6111P

HP6130-Biotin

Total Human IgM

HP6081P

HP6083-Biotin

Total Human IgM

HP6081P

HP6083-HRP

*The human IgG subclass enzyme immunoassays may be developed with a biotinylated poly-monoclonal anti-human IgG reagent, anti-kappa/lambda light chain reagent or a polyclonal mouse anti-human IgG reagent.

Capture antibodies are to be used at 10 micrograms per ml (1:200 dilution) in PBS-no protein for coating and detection antibodies are to be used at 1 microgram per ml (1:1000 dilution) in PBS-1% bovine serum albumin.

Horseradish Peroxidase Labeled Monoclonal Antibodies

Monoclonal antibodies specific for human IgG PAN (IgG1/2/3/4), IgA PAN (IgA1/2) and human IgM have been directly conjugated with horseradish peroxidase (HRP) using the Nakane/Kawaoi procedure. These antibodies are supplied at 1 mg/ml in 0.5 ml aliquots and should be diluted from 1:2000 to 1:8000 for use in immunoassays. These antibodies are being used in an internationally documented protocol for the measurement of human IgG, IgA and IgM antibodies specific for pneumococcal polysaccharides.

Clone

Specificity

Horseradish Peroxidase-conjugated

HP6043

anti-human IgG Fc

$130.00/0.5 mg

HP6083

anti-human IgM Fc

$130.00/0.5 mg

HP6123

anti-human IgA Fd

$130.00/0.5 mg