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For the Human Immunoglobulins

Human Isotype EIA	Capture Antibody	Detection Antibody
Total Human IgG	HP6046P	HP6043-HRP
Total Human IgG	HP6046P	PolyMono anti-human IgG *
Total Human IgG1	HP6069P	PolyMono anti-human IgG*
		Anti-Kappa/Lambda MAb *
Total Human IgG2	HP6002P	PolyMono anti-human IgG*
		Anti-Kappa/Lambda MAb *
Total Human IgG3	HP6047P	PolyMono anti-human IgG*
		Anti-Kappa/Lambda MAb *
Total Human IgG4	HP6025P	PolyMono anti-human IgG*
		Anti-Kappa/Lambda MAb *
Total Human IgE	HP6061P	HP6029-Biotin
Total Human IgA	HP6111P	HP6123-Biotin
		HP6123-HRP
Total Secretory IgA	HP6111P	HP6130-Biotin
Total Human IgM	HP6084P	HP6083-Biotin
Total Human IgM	HP6084P	HP6083-HRP

*The human IgG subclass enzyme immunoassays may be developed with a biotinylated poly-monoclonal anti-human IgG reagent, anti-kappa/lambda light chain reagent or a polyclonal mouse anti-human IgG reagent.

Capture antibodies are to be used at 10 micrograms per ml (1:200 dilution) in PBS-no protein for coating and detection antibodies are to be used at 1 microgram per ml (1:1000 dilution) in PBS-1% bovine serum albumin.

Horseradish Peroxidase Labeled Monoclonal Antibodies

Monoclonal antibodies specific for human IgG PAN (IgG1/2/3/4), IgA PAN (IgA1/2) and human IgM have been directly conjugated with horseradish peroxidase (HRP) using the Nakane/Kawaoi procedure. These antibodies are supplied at 1 mg/ml in 0.5 ml aliquots and should be diluted from 1:2000 to 1:8000 for use in immunoassays. These antibodies are being used in an internationally documented protocol for the measurement of human IgG, IgA and IgM antibodies specific for pneumococcal polysaccharides.

Clone	Specificity	Horseradish Peroxidase-conjugated
HP6043	anti-human IgG Fc	$165.00/0.5 mg
HP6083	anti-human IgM Fc	$165.00/0.5 mg
HP6123	anti-human IgA Fd	$165.00/0.5 mg